Coding
ecf16

Part:BBa_K2070001

Designed by: iGEM UT-Tokyo 2016   Group: iGEM16_UT-Tokyo   (2016-10-09)


ecf16

Ecf16 is an extracytoplasmic function (ECF) sigma factor taken from Pseudomonas entomophila used to activate the ecf16 promoter (BBa_K2070000). The sigma factor is found to be highly orthogonal to its corresponding promoter and can be inactivated by antisigma factor16 (BBa_K2070002).

Source

Pseudomonas entomophila L48

References

Rhodius, V. A., Segall-Shapiro, T. H., Sharon, B. D., Ghodasara, A., Orlova, E., Tabakh, H., Voigt, C. A. (2014, 04). Design of orthogonal genetic switches based on a crosstalk map of s, anti- s, and promoters. Molecular Systems Biology, 9(1), 702-702. doi:10.1038/msb.2013.58

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 177
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 372
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None